基于时序表达谱芯片挖掘鸡脂肪酸代谢关键调控基因

doi:10.11843/j.issn.0366-6964.2013.12.004

Abstract

Abstract:

Fatty acid metabolic products determine the flavor of chicken meat. Mining key genes regulating fatty acid metabolism has very important significance for improving meat quality and breeding new high quality broiler lines. The databases of KEGG and Wikipathway were used to analyze fatty acid metabolic networks, to find the key genes regulating fatty acid metabolism. The differential genes with co-expression of key genes in microarray in thigh muscle of chicken with different ages were filtered by Gene Spring(Pearson correlation coefficient over 0.9), then imported to co-expression network by Cytoscape. Finally, the functions of key regulatory genes were found by across-species retrieval，the node genes and related pathways of fatty acid metabolism were mined in chicken muscle tissue. The results showed that Ep300，Traf2 and Pak1 were node genes affecting fatty acid metabolism, and MAPK signaling pathway, ErbB signaling pathway and Herpes simplex infection pathway were critical pathways regulating fatty acid metabolism in chicken muscle tissue.These results provide basic information for studying the molecular mechanisms about fatty acid metabolism and for further screening quality meats genes in poultry.

CLC Number:

S831.2

WANG Hong-zhi, CHANG Guo-bin, MA Teng, ZHAI Fei, XIA Ming-xiu,LIU Lu, CHEN Jing, XU Lu, CHEN Guo-hong. Mining Key Genes of Chicken Fatty Acid Metabolism Based on Time Series Microarray[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1882-1890.

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Mining Key Genes of Chicken Fatty Acid Metabolism Based on Time Series Microarray

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